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Related Experiment Video

Updated: Jun 19, 2026

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

A single-base resolution map of an archaeal transcriptome.

Omri Wurtzel¹, Rajat Sapra, Feng Chen

¹Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

Genome Research

|November 4, 2009

Summary

This study maps the Sulfolobus solfataricus P2 transcriptome, revealing extensive noncoding RNAs and unique transcript features. These findings offer insights into RNA-based regulation in Archaea, distinct from Bacteria.

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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

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Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq

Published on: October 31, 2025

Area of Science:

Microbiology
Molecular Biology
Genomics

Background:

Archaea, the third domain of life, share traits with Bacteria and Eukarya.
They are crucial models for studying gene regulation and evolution.
Understanding archaeal transcription is key to deciphering fundamental biological processes.

Purpose of the Study:

To reconstruct the primary transcriptome of the model archaeon Sulfolobus solfataricus P2.
To map transcription start sites and operon structures at single-base-pair resolution.
To investigate noncoding RNA expression and RNA-based regulatory mechanisms in Archaea.

Main Methods:

High-throughput sequencing of cDNA to generate a high-resolution transcriptome map.
Analysis of over 625 million bases of sequence data.
Bioinformatic identification of transcriptional units, noncoding RNAs, and sequence motifs.

Main Results:

Detailed map of over 1000 transcriptional units in S. solfataricus P2.
Discovery of 310 expressed noncoding RNAs, including extensive cis-antisense transcripts.
Identification of unique transcript features, such as the lack of 5'-UTR sequences in most transcripts.
Uncovered internal hotspots for transcript cleavage and RNA destabilization motifs.

Conclusions:

Transcriptome sequencing is vital for understanding RNA-based regulation in Archaea and Bacteria.
Archaea exhibit extensive noncoding RNA expression and unique transcript structures, differing from Bacteria.
This study provides a foundational dataset for archaeal RNA biology and regulatory mechanisms.