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Related Experiment Videos

Yeast thioredoxin genes.

Z R Gan1

  • 1Department of Biological Chemistry, Merck Sharp & Dohme Research Laboratory, West Point, Pennsylvania 19486.

The Journal of Biological Chemistry
|January 25, 1991
PubMed
Summary
This summary is machine-generated.

Researchers identified two distinct yeast thioredoxin genes, thioredoxin I and thioredoxin II, through DNA sequencing. This discovery provides insights into eukaryotic thioredoxin evolution and function.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Thioredoxins are crucial redox proteins found across various organisms.
  • Conserved sequences suggest the presence of multiple thioredoxin genes in yeast.

Purpose of the Study:

  • To clone and sequence yeast thioredoxin genes.
  • To characterize the identified thioredoxin proteins and compare their sequences.

Main Methods:

  • Polymerase chain reaction (PCR) using conserved thioredoxin sequences.
  • Southern blot analysis to detect multiple gene copies.
  • DNA sequencing of cloned fragments to identify open reading frames.

Main Results:

  • Two yeast thioredoxin genes, encoding thioredoxin I (103 amino acids) and thioredoxin II (102 amino acids), were identified and sequenced.

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  • Both proteins share the active site sequence Cys-Gly-Pro-Cys.
  • Thioredoxin I and II exhibit 78% amino acid sequence identity and show greater similarity to mammalian thioredoxins than to bacterial or plant counterparts.

    • Conclusions:

    • This study reports the first cloning and sequencing of eukaryotic thioredoxin genes.
    • The findings suggest gene duplication and divergence as a mechanism for expanding the thioredoxin system in eukaryotes.
    • Yeast thioredoxins likely play conserved roles in cellular redox homeostasis, with evolutionary links to other species.