Related Experiment Video

Updated: Jun 19, 2026

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies
07:20

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

Cardiometabolic risk factors assessed by a finger stick dried blood spot method

Sanjay Kapur1, Sonia Kapur, David Zava

  • 1ZRT Laboratory, Beaverton, Oregon 97008, USA. skapur@zrtlab.com

Journal of Diabetes Science and Technology
|November 4, 2009
PubMed

Insights

Blood spot testing accurately measures key cardiometabolic risk factors like insulin, high-sensitivity C-reactive protein (hs-CRP), and triglycerides. This convenient method aids in screening and monitoring patients at risk for cardiovascular disease (CVD) and type 2 diabetes mellitus (T2DM).

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Biotechnology

Background:

  • Cardiovascular and metabolic risk factors are crucial for preventing cardiovascular disease (CVD) and type 2 diabetes mellitus (T2DM).
  • Blood spot technology provides an efficient and convenient approach for measuring these risk factors in at-risk populations.
  • Accessible methods for assessing cardiometabolic risk are vital for effective clinical management strategies.

Purpose of the Study:

  • To evaluate the efficacy of blood spot testing for measuring insulin, high-sensitivity C-reactive protein (hs-CRP), and triglycerides.
  • To compare the accuracy of blood spot measurements against conventional serum sample analysis.
  • To determine the utility of blood spot testing in cardiometabolic risk assessment.

Main Methods:

  • Insulin and hs-CRP were quantified in dried blood spots and serum using ELISA kits.
  • Triglyceride levels were determined enzymatically.
  • Blood spot assays were optimized for improved accuracy and reduced turnaround time.

Main Results:

  • Blood spot measurements showed strong correlations with serum levels for hs-CRP (r=0.99), triglycerides (r=0.94-0.95), and insulin (r=0.93-0.97).
  • The optimized blood spot assays demonstrated high accuracy compared to serum analysis.

Conclusions:

  • Blood spot testing is a reliable method for assessing insulin, hs-CRP, and triglycerides.
  • This technology can be valuable for cardiometabolic screening and monitoring of patients at risk for CVD, T2DM, or metabolic syndrome.
Abstract

Keywords:
C-reactive proteinCRPassay methodautomatedblood spotcardiometabolic panelcardiovascular diseasedried blood spothigh sensitivity CRPhs-CRPinsulinmetabolic syndromerisk factorsscreeningtriglyceridestype 2 diabetes

More Related Videos

Non-invasive Assessment of Microvascular and Endothelial Function05:41

Non-invasive Assessment of Microvascular and Endothelial Function

Published on: January 29, 2013

Pulse-Wave Velocity, Flow-Mediated Dilation, and Carotid Intima-Media Thickness to Assess Cardiovascular Risk in Population with Metabolic Syndrome06:04

Pulse-Wave Velocity, Flow-Mediated Dilation, and Carotid Intima-Media Thickness to Assess Cardiovascular Risk in Population with Metabolic Syndrome

Published on: September 27, 2024

Related Concept Videos

Type II Diabetes Mellitus III: Clinical Manifestations and Diagnosis01:25

Type II Diabetes Mellitus III: Clinical Manifestations and Diagnosis

Type 2 diabetes mellitus develops gradually and is often asymptomatic in early stages.Clinical ManifestationsWhen symptoms appear, they include fatigue, blurred vision, pruritus, delayed wound healing, and recurrent infections, particularly candidal infections. Peripheral neuropathy may present as numbness or tingling in the extremities. Classic hyperglycemia symptoms—polyuria, polydipsia, and polyphagia—are less common. Most patients are overweight and frequently have associated hypertension...
Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers01:19

Blood Studies for Cardiovascular System II: CRP, Hcy, and Cardiac Natriuretic Peptide Markers

Cardiac biomarkers are critical in diagnosing, prognosing, and managing cardiovascular diseases. Routine measurement of specific biomarkers such as B-type natriuretic peptide (BNP), C-reactive protein (CRP), and homocysteine (Hcy) is common practice in clinical settings to evaluate heart function and predict cardiovascular events.
These markers indicate stress or strain on the heart muscle:
Natriuretic Peptides (BNP)
Cardiac myocytes produce these hormones in response to ventricular stretching...
Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

Blood Studies for Cardiovascular System I: Cardiac Biomarkers

Cardiac biomarkers are enzymes, proteins, and hormones released into the blood when cardiac cells are injured. They are powerful tools for triaging.
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...
Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
Assessment of blood pressure in brachial artery(one-step method)01:15

Assessment of blood pressure in brachial artery(one-step method)

This procedural guide systematically measures blood pressure using an oscillometric digital sphygmomanometer, emphasizing accuracy, patient safety, and comfort.
Prepare for the Procedure:
Assessment of blood pressure in brachial artery(two-step method)01:23

Assessment of blood pressure in brachial artery(two-step method)

Measuring blood pressure is a fundamental skill in healthcare that aids in diagnosing and monitoring hypertension and other cardiovascular conditions. An aneroid sphygmomanometer, commonly used in clinical settings, offers a manual and precise method for blood pressure measurement. The technique for using this instrument involves specific steps that must be carefully executed to ensure accuracy. The following detailed description outlines a two-step technique for assessing blood pressure using...