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Updated: Jun 19, 2026

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
Published on: November 10, 2023
Example of use of taqman real-time RT-PCR to analyze bacterial gene transcript levels: Haemophilus influenzae
1University of Kentucky, Lexington, Kentucky, USA.
Abstract:
The protocol in this unit describes the steps for gene expression analysis of Haemophilus influenzae in broth culture. Steps for hot acid phenol RNA extraction, RNA cleanup, quantitation, assessment, and preparation for TaqMan qRT-PCR are described. The validation, setup, and analysis of TaqMan qRT-PCR experiments are discussed.
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