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Updated: Jun 19, 2026

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
Published on: December 10, 2014
Chelex-based DNA isolation procedure for the identification of microbial communities of eggshell surfaces
Antonio Manuel Martín-Platero1, Juan Manuel Peralta-Sánchez, Juan José Soler
1Departamento de Microbiología, Facultad de Ciencias, Universidad de Granada, 18071 Granada, Spain.
Abstract:
Microbial communities present on eggshell surfaces of wild birds are weakly studied, especially their influence on embryo infection and, thus, egg viability. Bacterial density of wild bird eggshells is very low, and most DNA extraction protocols are frequently unsuccessful. We have efficiently adapted a chelex-based DNA isolation method for 16S ribosomal gene amplification from the total communities of eggshell surfaces from six avian species.
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