Related Experiment Video
Updated: Jun 19, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Mechanisms of modulation of AMPA-induced Na+-activated K+ current by mGluR1
Evanthia Nanou1, Abdeljabbar El Manira
1Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Abstract:
Na(+)-activated K(+) (K(Na)) channels can be activated by Na(+) influx via ionotropic receptors and play a role in shaping synaptic transmission. In expression systems, K(Na) channels are modulated by G protein-coupled receptors, but such a modulation has not been shown for the native channels. In this study, we examined whether K(Na) channels coupled to AMPA receptors are modulated by metabotropic glutamate receptors (mGluRs) in lamprey spinal cord neurons. Activation of mGluR1 strongly inhibited the AMPA-induced K(Na) current. However, when intracellular Ca(2+) was chelated with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), the K(Na) current was enhanced by mGluR1. Activation of protein kinase C (PKC) mimicked the inhibitory effect of mGluR1 on the K(Na) current. Blockade of PKC prevented the mGluR1-induced inhibition of the K(Na) current, but did not affect the enhancement of the current seen in BAPTA. Together these results suggest that mGluR1 can differentially modulate AMPA-induced K(Na) current in a Ca(2+)- and PKC-dependent manner.
Related Concept Videos
Ligand-Gated Ion Channel Receptor: Gating Mechanism
G-Protein Gated Ion Channels
Sensory organs,...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Ligand-gated Ion Channels
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that include the...
GPCRs Regulate Adenylyl Cylase Activity
Two...
GPCR Desensitization

