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Updated: Jun 19, 2026

Identification of Mycobacterium Species by DNA Microarray Chip Method
Published on: June 24, 2025
Rapid method for identification of six common species of mycobacteria based on multiplex SNP analysis
Hongxiu Wang1, Jun Yue, Min Han
1Key Laboratory of Mycobacteria Tuberculosis, Shanghai Pulmonary Hospital affiliated to Tongji University, 507 Zhengming Rd., Shanghai 200433, People's Republic of China.
Abstract:
A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobacterial species by the multiplex SNaPshot method. Of the 468 mycobacterial isolates, 464 (99.15%) could be correctly identified by this assay. The multiplex SNaPshot technique is a promising discriminatory tool for rapid and accurate identification of frequently encountered clinical mycobacterial species.
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