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As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules. Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall of a...

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Related Experiment Video

Updated: Jun 19, 2026

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

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Structure-function insights into the yeast Dam1 kinetochore complex.

Eva Nogales¹, Vincent H Ramey

¹Department of Molecular and Cell Biology, UC Berkeley/Howard Hughes Medical Institute, UC Berkeley, Berkeley, CA 94720-3220, USA. enogales@lbl.gov

Journal of Cell Science

|November 6, 2009

Summary

This summary is machine-generated.

The Dam1 complex in budding yeast forms rings on microtubules, potentially driving chromosome segregation during cell division without extra energy. Its exact mechanism and broader applicability remain under investigation.

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Last Updated: Jun 19, 2026

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

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Published on: October 11, 2022

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Accurate chromosome segregation during cell division is vital for genome integrity.
Kinetochores mediate chromosome attachment to spindle microtubules.
The Dam1 complex (DASH) is crucial for chromosome segregation in budding yeast.

Purpose of the Study:

To review recent structure-function studies of the Dam1 complex.
To discuss the proposed model of Dam1 complex function in chromosome segregation.
To address controversies and questions regarding the Dam1 complex mechanism and its generality.

Main Methods:

In vitro studies of Dam1 complex interaction with tubulin.
Structural and biophysical analyses of the Dam1 complex.
Review of existing literature and models.

Main Results:

The Dam1 complex self-assembles into rings around microtubules.
These rings can track depolymerizing microtubule ends.
A model proposes Dam1 rings couple microtubule depolymerization to chromosome movement.

Conclusions:

The Dam1 complex's ring structure is key to its proposed function in chromosome segregation.
Controversies exist regarding the model's details and the Dam1 complex's evolutionary conservation.
Further structure-function studies are needed to fully understand this essential mitotic machinery.