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Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Engineering scaffold-free bone tissue using bone marrow stromal cell sheets.

Dongyang Ma1, Liling Ren, Yanpu Liu

  • 1Department of Oral and Maxillofacial Surgery, School of Stomatology, the Fourth Military Medical University, Chang Le 145 West Road, Xi'an, 710032, People's Republic of China.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|November 6, 2009
PubMed
Summary

This study developed scaffold-free bone tissue using bone marrow stromal cell (BMSC) sheets. These engineered tissues demonstrated successful bone formation and enhanced strength in vivo, offering a novel approach for bone repair.

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Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Exogenous scaffolds for bone tissue engineering present challenges like poor biological activity, immunogenicity, and variable degradation.
  • Existing methods often struggle with efficient cell attachment and integration.

Purpose of the Study:

  • To engineer large, scaffold-free bone tissue constructs using cell sheets derived from bone marrow stromal cells (BMSCs).
  • To evaluate the in vivo osteogenic potential and mechanical properties of these engineered bone tissues.

Main Methods:

  • Harvesting intact BMSC sheets via continuous culture and scraping.
  • Fabricating rolled cell sheets into large, scaffold-free constructs.
  • Implanting constructs into subcutaneous pockets of nude mice for in vivo evaluation.

Main Results:

  • BMSC sheets maintained osteogenic potential post-differentiation in vitro.
  • Computed tomography and histological analyses confirmed new bone formation in vivo.
  • Engineered bone constructs exhibited improved compressive strength.

Conclusions:

  • Scaffold-free BMSC sheets provide a viable strategy for generating functional 3D bone tissue.
  • This approach offers a promising alternative for bone defect repair, overcoming limitations of traditional scaffolds.