Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Duration of Outpatient Sodium Zirconium Cyclosilicate Therapy on Hyperkalemia-Related Health Care Resource Utilization: A Matched Cohort Analysis.

Kidney medicine·2026
Same author

Sodium Zirconium Cyclosilicate Duration and Hyperkalemia-Related Healthcare Encounters in Patients with Heart Failure and Cardio/Renal Dysfunction: The GALVANIZE Outcome Study.

Drugs - real world outcomes·2025
Same author

Renin-Angiotensin-Aldosterone System Inhibitor Dosing After Initiation of Outpatient Sodium Zirconium Cyclosilicate Therapy: The GALVANIZE RAASi Real-World Evidence Study.

Advances in therapy·2025
Same author

Occludin, caveolin-1, and Alix form a multi-protein complex and regulate HIV-1 infection of brain pericytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2020
Same author

Evidence of disulfide bond scrambling during production of an antibody-drug conjugate.

mAbs·2018
Same author

Rapid, automated characterization of disulfide bond scrambling and IgG2 isoform determination.

mAbs·2018

Related Experiment Video

Updated: Jun 19, 2026

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

Tagging for protein expression.

Arun Malhotra1

  • 1Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, Florida, USA.

Methods in Enzymology
|November 7, 2009
PubMed
Summary

Researchers often use protein tags for recombinant protein expression, aiding solubility and purification. This review surveys common affinity and solubility tags, providing guidance for selecting and using them effectively in protein production.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Tags are crucial for recombinant protein expression, enhancing solubility and enabling affinity purification.
  • A wide array of tags exists, presenting a challenge for researchers in selecting the optimal tag for protein production.
  • Effective tag selection is vital for successful protein expression and downstream applications.

Purpose of the Study:

  • To survey commonly used affinity and solubility tags for recombinant protein expression.
  • To provide guidance on the selection and application of various protein tags.
  • To assist researchers in navigating the choices available for protein tagging strategies.

Main Methods:

  • Literature review of established and novel protein tags.

More Related Videos

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research
07:09

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research

Published on: March 1, 2024

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Related Experiment Videos

Last Updated: Jun 19, 2026

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research
07:09

Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research

Published on: March 1, 2024

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

  • Categorization of tags based on function (affinity, solubility) and properties.
  • Analysis of tag performance characteristics and experimental considerations.

    • Main Results:

    • Detailed overview of popular affinity tags (e.g., His-tag, GST-tag, MBP-tag).
    • Discussion of solubility tags and their mechanisms (e.g., SUMO, NusA).
    • Comparative analysis of tag advantages, disadvantages, and purification strategies.

    Conclusions:

    • The choice of tag significantly impacts recombinant protein yield, solubility, and purity.
    • Understanding tag characteristics is essential for optimizing protein expression and purification protocols.
    • This survey aims to facilitate informed decisions in designing protein expression constructs for diverse research needs.