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Detergent Purification of Membrane Proteins

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Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

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Related Experiment Video

Updated: Jun 19, 2026

Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

Purification of the Sarco-Endoplasmic Reticulum Ca²⁺-ATPase from Rabbit Muscle

Published on: March 21, 2025

Purification of membrane proteins.

Sue-Hwa Lin¹, Guido Guidotti

¹Department of Molecular Pathology, University of Texas, Houston, Texas, USA.

Methods in Enzymology

|November 7, 2009

Summary

Purifying membrane proteins requires careful detergent selection. This overview covers plasma membrane isolation and how detergents impact membrane protein purification for better characterization.

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Membrane proteins are crucial for cellular functions.
Their low abundance and requirement for detergents make purification challenging.
Detergent choice is critical for successful membrane protein isolation.

Purpose of the Study:

To provide an overview of plasma membrane isolation techniques.
To discuss the selection of detergents for membrane protein solubilization.
To explain the impact of detergent choice on purification outcomes.

Main Methods:

Isolation of plasma membranes from cells.
Screening of various detergents for protein solubilization.
Analysis of detergent effects on protein stability and purity.

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Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

Published on: December 3, 2010

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

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Purification of the Sarco-Endoplasmic Reticulum Ca2+-ATPase from Rabbit Muscle

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Published on: March 21, 2025

Expression, Detergent Solubilization, and Purification of a Membrane Transporter, the MexB Multidrug Resistance Protein

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Published on: December 3, 2010

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

Published on: July 4, 2016

Main Results:

Successful isolation of plasma membranes is achievable.
Different detergents exhibit varying efficiencies in solubilizing membrane proteins.
Detergent selection significantly influences the success of subsequent purification steps.

Conclusions:

Effective membrane protein purification hinges on appropriate detergent selection.
Understanding detergent-protein interactions is key to characterizing these vital biomolecules.