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Updated: Jun 19, 2026

Cell-Type Specific Protein Purification and Identification from Complex Tissues Using a Mutant Methionine tRNA Synthetase Mouse Line
Published on: April 13, 2022
Important but little known (or forgotten) artifacts in protein biochemistry
1McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Abstract:
Many subtle and sometimes obscure artifacts exist that can have major effects on the outcomes of otherwise carefully performed experiments. This chapter focuses on a few of these artifacts involved in processes such as SDS gel sample preparation, buffers, Ni-chelate affinity and ion-exchange chromatography, urea preparation, use of plasticware, and filtration of protein samples.
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