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Published on: March 18, 2019
Human platelets perfusion through isolated guinea-pig heart: the effects on coronary flow and oxidative stress
Slobodan Novokmet1, Vladimir Lj Jakovljevic, Slobodan Jankovic
1Department of Pharmacy, Faculty of Medicine, University of Kragujevac, Serbia.
Insights
Human platelet-rich plasma (PRP) affects coronary flow and oxidative stress. Activated platelets from acute myocardial infarction patients significantly reduced coronary flow and oxidative stress markers in isolated guinea-pig hearts.
Area of Science:
- Cardiovascular Physiology
- Platelet Biology
- Oxidative Stress Research
Background:
- Platelets play a crucial role in cardiovascular health and disease.
- Understanding platelet-endothelial interactions is vital for managing coronary vascular conditions.
- Oxidative stress is a key factor in the pathophysiology of acute myocardial infarction.
Purpose of the Study:
- To investigate the impact of human platelet-rich plasma (PRP) on coronary flow (CF) and oxidative stress markers.
- To compare the effects of PRP from healthy donors versus patients with acute myocardial infarction (AMI).
- To assess the role of nitric oxide synthase (NOS) inhibition in mediating these effects.
Main Methods:
- Isolated guinea-pig hearts were perfused using the Langendorff technique at varying coronary perfusion pressures (CPP).
- Coronary venous effluent was analyzed for oxidative stress markers: nitrite (NO production), superoxide anion (O2(-)), and TBARS (lipid peroxidation).
- Hearts were perfused with PRP from healthy volunteers and AMI patients, with and without NOS inhibition (L-NAME).
Main Results:
- Perfusion with both PRP and PRP (AMI) reduced CF and oxidative stress markers.
- PRP (AMI) caused a more pronounced reduction in CF compared to PRP from healthy donors.
- Oxidative stress markers were significantly decreased only with PRP (AMI), an effect potentiated by NOS inhibition.
Conclusions:
- Both non-activated and activated platelets interact with the coronary endothelium.
- Activated platelets, particularly from AMI patients, exert a more significant influence on coronary flow and oxidative stress.
- These findings highlight the differential impact of platelet activation status on coronary vascular function.
Abstract:
Present study was designed to evaluate effect of perfusion with human platelets reach plasma (PRP) on coronary flow (CF) and oxidative stress markers in coronary vascular bed of the isolated guinea-pig heart. In coronary venous effluent the following oxidative stress markers were estimated: nitrite as a measure of nitric oxide (NO) production, superoxide anion (O2(-)), and index of lipid peroxidation (TBARS). Isolated guinea-pig hearts (n = 6, b.m. 250-300 g) were perfused according to a Langendorff's technique at different (30, 70, and 120 cmH2O) coronary perfusion pressures (CPP). Samples were collected at control conditions and during perfusion with platelets rich plasma (PRP) obtained either from healthy volunteers or from patients with acute myocardial infarction (PRP (AMI)) with/or without previous inhibition of NO synthase (NOS) by Nomega-nitro-L-arginine monomethyl ester (L-NAME, 30 micromol/l). PRP and PRP (AMI) perfusion induced reduction of CF and all evaluated oxidative stress parameters. The reduction of CF was more potentiated in PRP (AMI) as in PRP group, while oxidative stress parameters where significantly decreased only in PRP (AMI). In addition, previous blockade of NOS by L-NAME potentiated these effects only in PRP (AMI) group. It can be concluded that non-activated and activated platelets interact with coronary endothelium in similar way, with more significant influence of activated platelets on CF and oxidative stress markers.
