Related Experiment Video
Updated: Jun 18, 2026

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
Published on: September 10, 2021
[Identification on Syngnathus and its adulterants with random amplified polymorphic DNA]
Yan Wu1, Jia Liu, Mengyue Wang
1School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Objective:
To study the identification method of Syngnathus and its adulterants.
Method:
Random amplified polymorphic DNA (RAPD) was used to construct a dendrogram by UPGMA method based on Nei & Li's coefficient and a genetic affinity pattern for Syngnathus acus, Solenognathus hardwickii, Syngnathoides biaculeatus, Trachyrhamphus serratus, Halicampus koilomatodon, Microphis boaja.
Result:
Four primers, LJ04, LJ09, LJ16 and LJ19, from 18 random primers were used in the dendrogram which can differentiate Syngnathus in genus level and showed a great consistence with the appearance identification. The genetic affinity pattern based on primers LJ09 and LJ19 could be used to identify Syngnathus from its adulterants.
Conclusion:
RAPD is suitable to identify Syngnathus and its adulterants.
Related Concept Videos
Methods of Classification and Identification
Modern Molecular Taxonomy

