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[Agrobacterium tumefaciens mediated Chitinase and beta-1,3-glucanase gene transformation for Pinellia ternata]
Bo Jin1, Fusheng Jiang, Meirong Yu
1Life and science college of Zhejiang Chinese Medial University, Hangzhou 310053, China. jinbont@sina.com
Objective:
To obtain transgenic Pinellia ternata plants resistant to fungus by transfer Chitinase and beta-1,3-Glucanase gene from Trichoderma harzianum.
Method:
Using hygromycin phosphotransferase as the selection marker, the Chitinase gene (ech42), beta-1,3-Glucanase gene (gluc78) and both gene pCAMBIA(ech42 + gluc78) driven by CaMV35S promoter were transferred into P. ternata callus via Agrobacterium-mediated transformation.
Result:
PCR results confirmed that the regenerants were identified to be transgenic lines and the RT-PCR results confirmed that foreign genes construction were transfer to mRNA. Two foreign genes were inherited stably to T5 generation according to PCR results of the lines.
Conclusion:
The results showed that chitinase gene ech42 and beta-1, 3-glucanase gene gluc78 respectively or together introducing and co-integrating into P. ternata
