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[Conditions for extraction of ergosterol from fruits of Ganoderma lucidum using supercritical fluid extration]
Shihua Song1, Xiaobin Jia, Yan Chen
1Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Objective:
To study the feasibility of supercritical fluid extraction (SFE) for extraction of ergosterols from the fruits of Ganoderma lucidum.
Method:
Optimum extraction conditions were studied by orthogonal tests, the ergosterol were analyzed by RP-HPLC.
Result:
The optimal extraction conditions were pressure as 20 MPa, temperature at 45 degrees C, using ethanol as modifier carrier at the volume of 1 mL x g(-1), and 1.5 hour of extraction time.
Conclusion:
SFE has the superiority of adjustable polarity, and has the ability of extracting ergosterol from fruits of Ganoderma lucidum.
