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Updated: Jun 18, 2026
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
Published on: July 13, 2011
[Protective effects of catalpol against H2O2-induced oxidative damage in astrocytes]
Ziqiang Zhang1, Yumei Liu, Bangqun Xue
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. zzq828929@163.com
Objective:
To study the protective effects of catalpol against H2O2-induced oxidative damage in astrocytes.
Method:
Astrocytes were pretreated with 1, 10, 100 micromol x L(-1) catalpol and then exposed to H2O2. Effects of catalpol on cell morphous were observed by inverted microscope. Cell viability were detected by MTT assay. LDH content in the cell culture fluid, and intracellular T-SOD, GSH-Px activity and MDA content were assessed by colorimetric method.
Result:
The results showed that catalpol evidently ameliorated cell morphous, improved cell viability, and maintained integrality of cell membrane. Furthermore, catalpol could significantly inhibit the decrease of intracellullar T-SOD and GSH-Px activity, and the increase of MDA content.
Conclusion:
Catalpol have protective effects against H2O2-induced oxidative damage in astrocytes.
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