Related Experiment Video

Updated: Jun 18, 2026

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

[Features of binding of proflavine to DNA at different DNA-ligand concentration ratios]

E G Berezniak, N A gladkovskaia, A S Khrebtova

    Biofizika
    |November 10, 2009
    PubMed

    More Related Videos

    High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy06:38

    High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

    Published on: February 7, 2019

    Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes05:33

    Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes

    Published on: July 5, 2024

    Related Concept Videos

    Labeling DNA Probes03:31

    Labeling DNA Probes

    DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
    Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
    The Equilibrium Binding Constant and Binding Strength02:18

    The Equilibrium Binding Constant and Binding Strength

    The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
    Cooperative Binding of Transcription Regulators02:13

    Cooperative Binding of Transcription Regulators

    Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
    Single-Strand DNA Binding Proteins01:03

    Single-Strand DNA Binding Proteins

    For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...