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Updated: Jun 18, 2026

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
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Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage.

Wen Lo1, Yu-Lin Chang, Jia-Shiu Liu

  • 1National Taiwan University, Department of Physics and Center for Quantum Science and Engineering, Tainan, Taiwan.

Journal of Biomedical Optics
|November 10, 2009
PubMed
Summary

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Multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) imaging reveal thermal damage in bovine corneas. FWSHG intensity decay and FWSHG/BWSHG image convergence are sensitive indicators of corneal thermal damage.

Area of Science:

  • Biomedical Optics
  • Ophthalmology
  • Materials Science

Background:

  • Thermal damage to the cornea can impair vision.
  • Accurate characterization of thermal damage is crucial for clinical assessment and treatment.
  • Non-invasive imaging techniques offer potential for evaluating corneal structural integrity.

Purpose of the Study:

  • To qualitatively and quantitatively characterize thermal damage in ex vivo bovine corneas.
  • To investigate the utility of multiphoton autofluorescence (MAF) and forward/backward second-harmonic generation (FWSHG/BWSHG) imaging for assessing thermal injury.
  • To correlate imaging signal changes with increasing thermal insult.

Main Methods:

  • Ex vivo bovine corneas were subjected to controlled thermal treatments (37-90°C).

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  • Multimodal imaging including MAF, FWSHG, and BWSHG was employed to capture structural alterations.
  • Image correlation analysis was performed between FWSHG and BWSHG signals.

    • Main Results:

    • Increasing thermal damage led to significant increases in image correlation between FWSHG and BWSHG.
    • MAF and BWSHG signal intensities showed preliminary correlation with thermal damage extent.
    • FWSHG intensity decay and the convergence of FWSHG/BWSHG images proved to be the most sensitive indicators of thermal damage.

    Conclusions:

    • Multimodal SHG and MAF imaging can effectively characterize thermal damage in corneas.
    • FWSHG intensity decay and FWSHG/BWSHG image correlation provide sensitive quantitative measures of corneal thermal injury.
    • These advanced imaging techniques show promise for non-invasive assessment of thermal damage in ocular tissues.