DNA microarrays provide new options for allergen testing

Microarray studies are increasingly used for toxicological research and even for the development of new toxicological test methods. Since gene-expression changes in cultured cells can be conveniently measured with microarrays, this method might be of use for in vitro toxicity testing, for example, in the field of contact sensitization. Allergic contact dermatitis, the clinical manifestation of contact sensitization, may occur when sensitizing chemicals enter the skin and get in contact with epidermal and dermal antigen-presenting cells. The resulting maturation process in these cells can be measured by employing gene-expression analysis. Biomarkers currently known seem to be insufficient to identify all kinds of contact sensitizers, which may partly activate different signaling pathways (e.g., metal or organic sensitizers). Therefore, genome-wide screenings using whole-genome DNA microarrays and extensive data analysis can be performed in order to identify additional genes. Ultimately, marker genes detected in whole-genome experiments can be included in small-scale-targeted microarrays in order to establish the final test method.