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Developing HiPSC Derived Serum Free Embryoid Bodies for the Interrogation of 3-D Stem Cell Cultures Using Physiologically Relevant Assays
Published on: July 20, 2017
Novel embryoid body-based method to derive mesenchymal stem cells from human embryonic stem cells
Eun Ju Lee1, Ha-Neul Lee, Hyun-Jae Kang
1National Research Laboratory for Cardiovascular Stem Cells and IRICT, Seoul National University Hospital, Seoul, Republic of Korea.
Tissue Engineering. Part A
|November 10, 2009
Summary
A novel method efficiently derives human mesenchymal stem cells (hMSCs) from human embryonic stem cells (hESCs). This technique avoids tumorigenicity and ensures consistent cell characteristics for therapeutic applications.
Area of Science:
- Stem cell biology
- Regenerative medicine
Background:
- Human embryonic stem cells (hESCs) pose tumorigenicity and rejection risks for therapy.
- Adult human mesenchymal stem cells (hMSCs) exhibit variable characteristics.
Purpose of the Study:
- To develop a new, safe, and reproducible method for deriving hMSCs from hESCs.
Main Methods:
- hESCs were differentiated into hMSCs using embryoid body formation and stage-specific media.
- No xenogenic feeders or mechanical selection were employed.
- Three hESC lines (SNUhES3, CHA3-hESC, H9) were used.
Main Results:
- Derived cells exhibited hMSC morphology and stable growth for over 35 passages.
- Cells displayed characteristic hMSC immunophenotypes (CD73+, CD90+, CD105+) and multipotency.
- Transplantation into immunodeficient mice showed no tumorigenicity.
Conclusions:
- The embryoid body-based method offers a simple, safe, and reproducible way to derive hMSCs from hESCs.
- This approach can yield consistent and non-tumorigenic hMSCs for therapeutic use.
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