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Updated: Jun 18, 2026

Sample Drift Correction Following 4D Confocal Time-lapse Imaging
Published on: April 12, 2014
[A drift induced by the acquisition of a video system linked to a microscope]
1Unité de biologie de la reproduction et d'assistance médicale à la procréation, laboratoire de biologie polyvalente, hôpital Louis-Pasteur, 4, rue Claude-Bernard, 28630 Le Coudray, France. alaudat@ch-chartres.fr
Abstract:
Further to the acquisition of a new material consisting in a microscope and its video equipment, we report the example of some drift with regards to written, updated and validated procedures. Indeed, once the new material was fully operating, depending on the operator, the (spermocytogrammes) morphology was realized either in oculars or by using the video screen. The statistical study performed on the results from 50 patients showed a significant difference in the results of the appreciation of the microcephalous and the macrocephalous according to the mode of observation that had been used. As an outcome of this study, we insisted that everyone within the team should work consistently and comply with the current procedures.

