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PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions
Published on: July 27, 2017
Surface modification with BSA blocking based on in situ synthesized gold nanoparticles in poly(dimethylsiloxane)
Da-He Fan1, Shi-Wei Yuan, Yong-Miao Shen
1School of Chemical and Biological Engineering, Yancheng Institute of Technology, Yancheng 224003, PR China.
Colloids and Surfaces. B, Biointerfaces
|November 10, 2009
Summary
Researchers developed a stable poly(dimethylsiloxane) (PDMS) microchannel using gold nanoparticles to prevent protein adsorption. This innovation enhances microchip performance for applications like electrophoretic separation.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Poly(dimethylsiloxane) (PDMS) is widely used in microfluidic devices.
- Protein adsorption on PDMS surfaces can interfere with microchip performance.
- Developing effective surface modifications for PDMS is crucial for reliable microfluidic applications.
Purpose of the Study:
- To create a stable, protein-repellent surface for PDMS microchannels.
- To utilize in situ synthesized poly(dimethylsiloxane)-gold nanoparticle composite films for surface modification.
- To evaluate the performance of the modified microchannels in suppressing protein adsorption and enabling electrophoretic separation.
Main Methods:
- Synthesis of PDMS-gold nanoparticle composite films for in situ coating.
- Surface characterization using contact angle measurements.
- Assessment of electroosmotic flow (EOF) stability and reproducibility.
- Evaluation of protein adsorption using charge-coupled device (CCD) imaging and electrophoretic separation of FITC-labeled myoglobin.
Main Results:
- The modified PDMS surface demonstrated significantly reduced hydrophobicity, with a water contact angle of 45.2 degrees compared to 88.5 degrees for native PDMS.
- The coated microchannels exhibited stable and reproducible electroosmotic flow (EOF) behavior.
- CCD imaging showed no detectable adsorption of FITC-labeled myoglobin onto the modified surface.
- Effective electrophoretic separation of myoglobin was achieved in the modified microchip.
Conclusions:
- In situ synthesized PDMS-gold nanoparticle composite films provide a stable and effective surface modification for PDMS microchannels.
- The modified surface successfully suppresses protein adsorption, enhancing microchip reliability.
- This approach offers a promising strategy for improving microfluidic device performance in biochemical analyses and separations.
