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A Murine Orthotopic Bladder Tumor Model and Tumor Detection System
Published on: January 12, 2017
A novel detection system for submicroscopic human metastases in athymic mice
B A McKenzie1, A Barrieux, N M Varki
1Department of Medicine and Pathology, University of California Medical Center, San Diego 92093.
Cancer Communications
|January 1, 1991
Summary
Researchers created a sensitive assay to detect human tumor DNA in mouse organs. This method can identify human neoplasm metastases in athymic mouse models with high accuracy.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Athymic mouse models are crucial for studying human cancers.
- Accurate detection of human tumor DNA in mouse models is essential for research.
- Current methods may lack the sensitivity to detect minimal residual disease.
Purpose of the Study:
- To develop a highly sensitive assay for detecting human tumor DNA in athymic mouse organs.
- To quantify human tumor DNA presence in various mouse tissues.
- To validate the assay's utility in identifying human neoplasm metastases.
Main Methods:
- Preparation of genomic DNA from human lung carcinoma cell lines and athymic mouse lungs.
- Slot-blotting of DNA mixtures onto nylon filters.
- Hybridization with labeled, human-specific Alu sequences.
Main Results:
- The assay demonstrated high sensitivity, detecting the equivalent of one human cell per 2000 mouse cells.
- The system accurately differentiated between human and mouse DNA.
- Successful detection of human tumor DNA in athymic mouse organs was achieved.
Conclusions:
- A sensitive and accurate assay system for detecting human tumor DNA in athymic mouse organs has been established.
- This assay is valuable for confirming the presence or absence of human neoplasm metastases.
- The developed assay enhances the reliability of the athymic mouse model system in cancer research.
