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Published on: March 5, 2017
Detection of 5-lipoxygenase activity in the liverwort Marchantia polymorpha L
Hirosuke Kanamoto1, Miho Takemura, Kanji Ohyama
1Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Ishikawa, Japan.
Abstract:
We detected 5-LOX (arachidonate 5-lipoxygenase) in the homogenate of Marchantia polymorpha by spectrophotometry and mass spectrometry. LC/MS/MS analysis indicated that the liverwort 5-LOX produced 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid (5-HPETE) with arachidonic acid as a substrate. The 5-LOX activity showed a Ca(2+) response, as demonstrated for human 5-LOX. These findings suggest that the liverwort utilizes an arachidonate cascade in a defense signal response.
