Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 18, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Digital microfluidics for automated proteomic processing.

Mais J Jebrail1, Vivienne N Luk, Steve C C Shih

  • 1Department of Chemistry, University of Toronto, Canada.

Journal of Visualized Experiments : Jove
|November 10, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A digital microfluidic platform for cell-based non-invasive testing.

iScience·2026
Same author

DROP-LCMS for wastewater surveillance of viral disease.

Lab on a chip·2026
Same author

Sequential transcriptional waves and NF-κB-driven chromatin remodeling direct drug-induced dedifferentiation in cancer.

Nature communications·2026
Same author

μPharma: A microfluidic, AI-driven pharmacotyping platform for single-cell drug sensitivity prediction in leukemia.

Med (New York, N.Y.)·2026
Same author

A combined digital microfluidic test for assessing infection and immunity status for viral disease in saliva.

Lab on a chip·2025
Same author

A compartmentalization-free microfluidic digital assay for detecting picogram levels of protein analytes.

Lab on a chip·2025
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

A novel digital microfluidics (DMF) method automates protein processing for clinical proteomics. This technique integrates multiple steps, offering a promising advancement for biomarker discovery in disease diagnosis and prognosis.

Area of Science:

  • Proteomics
  • Biochemistry
  • Microfluidics

Background:

  • Clinical proteomics aims to discover disease biomarkers for early diagnosis and prognosis.
  • Current methods require extensive protein extraction and processing from heterogeneous biological fluids.
  • Existing techniques often rely on manual pipetting or robotics, which can be cumbersome.

Purpose of the Study:

  • To introduce a new digital microfluidics (DMF) based method for integrated protein processing in clinical proteomics.
  • To demonstrate the automation of sequential biochemical assays on a microscale.
  • To present DMF as a potential advancement over conventional clinical proteomics workflows.

Main Methods:

  • Developed a digital microfluidics (DMF) system for manipulating nanoliter-microliter droplets on an open surface.

More Related Videos

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Related Experiment Videos

Last Updated: Jun 18, 2026

Digital Microfluidics for Automated Proteomic Processing
10:55

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
12:21

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

  • Integrated key protein processing steps: extraction, resolubilization, reduction, alkylation, and enzymatic digestion.
  • Utilized an array of electrodes to control droplet movement, merging, mixing, and splitting via electrostatic forces.

    • Main Results:

    • Successfully integrated multiple essential protein processing steps into a single automated DMF workflow.
    • Demonstrated the capability of DMF to perform rapid, sequential, and miniaturized biochemical assays.
    • Showcased DMF's potential for efficient handling of complex proteomic sample preparation.

    Conclusions:

    • Digital microfluidics offers a powerful platform for automating complex biochemical assays in clinical proteomics.
    • This DMF method represents a significant advance over traditional manual or robotic approaches.
    • The integrated DMF system has the potential to become a valuable tool for biomarker discovery and disease diagnostics.