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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jun 18, 2026

$Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing$

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Nanophotonics: lasers go beyond diffraction limit

Martin T Hill¹

¹COBRA Research Institute, Technical University of Eindhoven, Eindhoven, The Netherlands. m.t.hill@tue.nl

Nature Nanotechnology

|November 10, 2009

Summary

No abstract available in PubMed .

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