Over-expression and characterization of recombinant prefoldin from hyperthermophilic archaeum Pyrococcus furiosus in
Biotechnology Letters
|November 10, 2009
Abstract:
Prefoldin is a hexameric chaperone that has been identified in eukaryotic cells and in Archaea. E. coli cells over-expressing the prefoldin gene from the hyperthermophilic, archaeum, Pyrococcus furiosus, grew well at 48 degrees C while control cells were unable to grow above 46 degrees C. The isolated and purified Pfu-prefoldin (specially the beta subunit and the prefoldin) thermally protected the activity of lysozyme.
