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Novel monoclonal antibodies against Menangle virus nucleocapsid protein

Aurelija Zvirbliene1, Indre Kucinskaite-Kodze, Mindaugas Juozapaitis

  • 1Institute of Biotechnology, V. Graiciuno 8, 02241 Vilnius Lithuania. azvirb@ibt.lt

Archives of Virology
|November 10, 2009
PubMed

Insights

Three new monoclonal antibodies (MAbs) were developed to detect Menangle virus (MenV), a cause of pig reproductive disease. These MAbs show promise for accurate immunohistological diagnosis of MenV infections in pigs.

Area of Science:

  • Veterinary Virology
  • Immunology
  • Molecular Biology

Background:

  • Menangle virus (MenV), a Paramyxoviridae family member, causes significant reproductive issues in pigs.
  • Accurate diagnostic tools are crucial for controlling MenV outbreaks.
  • Existing diagnostic methods may lack specificity or sensitivity.

Purpose of the Study:

  • To develop specific monoclonal antibodies (MAbs) for Menangle virus detection.
  • To characterize the reactivity and epitope mapping of newly generated MAbs.
  • To evaluate the utility of MAbs in immunohistological diagnosis of MenV infection.

Main Methods:

  • Generation of MAbs by immunizing mice with recombinant MenV nucleocapsid (N) protein.
  • Epitope mapping using truncated MenV N proteins.
  • Immunohistochemical staining of pig brain tissue from a field case.

Main Results:

  • Three IgG1 MAbs were successfully generated against the MenV N protein.
  • Two MAbs recognized epitopes within amino acid residues 430-460, and one recognized 460-490.
  • All MAbs specifically detected MenV in infected piglet brain tissue.

Conclusions:

  • Novel MAbs offer specific detection of Menangle virus.
  • These MAbs are valuable tools for the immunohistological diagnosis of MenV infection in pigs.
  • Further application in diagnostic assays can aid in disease management.

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