The effect of interfering ions on search algorithm performance for electron-transfer dissociation data
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Updated: Jun 18, 2026

Thermochemical Studies of Ni(II) and Zn(II) Ternary Complexes Using Ion Mobility-Mass Spectrometry
Published on: June 8, 2022
David M Good1, Craig D Wenger, Joshua J Coon
1Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Abstract:
Collision-activated dissociation and electron-transfer dissociation (ETD) each produce spectra containing unique features. Though several database search algorithms (e.g. SEQUEST, MASCOT, and Open Mass Spectrometry Search Algorithm) have been modified to search ETD data, this consists chiefly of the ability to search for c- and z(*)-ions; additional ETD-specific features are often unaccounted for and may hinder identification. Removal of these features via spectral processing increased total search sensitivity by approximately 20% for both human and yeast data sets; unique peptide identifications increased by approximately 17% for the yeast data sets and approximately 16% for the human data set.
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