Related Experiment Video
Updated: Jun 18, 2026
A Fluorescence-based Protocol for Preliminary Screening of Protein Synthesis Inhibitors from Natural Sources
Published on: January 27, 2026
[Expression of truncated fragment of human OPG in CHO-DHFR- cells and its bioactivity characterization]
Bo-hong Kang1, Xiao-yi Zang, Lan-ying Li
1Key Laboratory of Hormones & Development of Ministry of Health, Metabolic Disease Hospital and Endocrinology Institute of Tianjin Medical University, Tianjin 300070, China. kanbh606@sohu.com
Aim:
To obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR(-) cells.
Methods:
The recombinant vector pcDNA3.1/DHFR-TOPG was constructed and transfected into CHO-DHFR(-) cells by the directions of LipofectAMINE 2000 for stable expression. The stable expression cell strains were screened by selective medium IMDM with 50 mL/L FCS, then serially passed in methotraxate (MTX) for gene amplification. The expression were analyzed by ELISA and RT-PCR. At last, the bioactivity analysis was performed in vitro.
Results:
The expression level of recombinant truncated human OPG was up to 6 mg/L x 72 h, and it had significant suppression effect on the formation of OLC (P<0.05).
Conclusion:
Recombinant truncated human OPG has high expression and bioactivity. The results make it possible for further studying and clinical implying of OPG.
