Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Supportive care needs, decision-making participation attitudes, and quality of life in young and middle-aged Chinese patients with cancer: A latent profile and mediation analysis.

Asia-Pacific journal of oncology nursing·2026
Same author

Molecular and clinical characterization of respiratory syncytial virus genotypes in a medical centre in Central Taiwan across the COVID-19 era.

Journal of infection and public health·2026
Same author

A hybrid radiomics framework integrating genetic algorithm-optimized random forest for preoperative identification of Luminal B breast cancer and Ki-67 prediction: A multicenter study.

The ultrasound journal·2026
Same author

Elucidating structure-function relationships in the mammalian nucleolus.

Nature reviews. Molecular cell biology·2026
Same author

Parental involvement in neonatal decision-making: a narrative review.

Frontiers in pediatrics·2026
Same author

DockingDB: An online reverse-docking database for plant hormone research.

Plant communications·2026

Related Experiment Video

Updated: Jun 18, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Theoretical methods for identifying important functional genes in bacterial genomes.

Junxiang Gao1, Ling-Ling Chen

  • 1School of Information and Communication Engineering, Beijing University of Posts and Telecommunications, Beijing 100876, PR China.

Research in Microbiology
|November 11, 2009
PubMed
Summary

This review summarizes computational methods for identifying key bacterial functional genes, including essential, highly expressed, and horizontally transferred genes. These methods are crucial for understanding bacterial survival and pathogenicity by exploring bacterial genomes.

More Related Videos

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Related Experiment Videos

Last Updated: Jun 18, 2026

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
12:24

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

Published on: July 21, 2014

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Area of Science:

  • Genomics
  • Microbiology
  • Bioinformatics

Background:

  • Functional genes like essential, highly expressed, and horizontally transferred genes are vital for bacterial survival and pathogenicity.
  • Identifying these genes aids in understanding bacterial biology and evolution.

Purpose of the Study:

  • To review and summarize current computational methods for identifying key functional genes in bacterial genomes.
  • To highlight the significance of these methods in bacterial genome exploration.

Main Methods:

  • Literature review of computational approaches.
  • Analysis of methods for identifying essential genes.
  • Review of techniques for detecting highly expressed genes.
  • Examination of strategies for finding horizontally transferred genes.

Main Results:

  • Overview of diverse computational tools and algorithms.
  • Discussion of the strengths and limitations of various identification methods.
  • Emphasis on the importance of integrating multiple approaches.

Conclusions:

  • Computational methods are essential for accurate identification of functional genes in bacterial genomes.
  • Further development of these methods will enhance our understanding of bacterial traits and functions.
  • This review provides a valuable resource for researchers in bacterial genomics.