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Published on: February 3, 2020
A new methodology for processing mouthparts of hemipterans (Hemiptera) for microscopic studies
Rogério A Depieri1, Célia G T J Andrade, Antônio R Panizzi
1Department of Zoology, Federal University of Paraná, P.O. Box 19020, Curitiba, PR, 81531-980, Brazil.
Abstract:
A new methodology has been developed to produce sections as thin as 5 microm of stink bug (Heteroptera: Pentatomidae) mouthparts for morphological studies. Heads of the southern green stink bug, Nezara viridula (L.) (Heteroptera: Pentatomidae) were fixed on Bouin solution, and treated with EDTA (ethylenediamine tetraacetic acid 7%) in an ice bath within a microwave oven for 0.5, 1, 2, 3, 4, and 5 h. The chitin structures and labium soft parts were well preserved after 0.5h EDTA treatment. Longer times of exposure to EDTA and microwave irradiation caused damage to mouthpart structures.

