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The MiST2 database: a comprehensive genomics resource on microbial signal transduction
1Agile Genomics LLC, Mount Pleasant, SC 29466, USA. ulrich.luke+sci@gmail.com
Nucleic Acids Research
|November 11, 2009
Summary
The MiST2 database catalogs microbial signal transduction proteins, crucial for understanding cellular activities and pathogen virulence. This updated resource enhances microbial genomics research with new features and expanded data.
Area of Science:
- Microbiology
- Bioinformatics
- Genomics
Background:
- Signal transduction systems regulate essential cellular activities in microbes, including metabolism, development, and virulence.
- Understanding these protein networks is vital for biomedical discovery, particularly in human pathogens.
- The MiST2 database provides a catalog of these critical signaling proteins in microbial genomes.
Purpose of the Study:
- To introduce MiST2, an updated database for identifying and cataloging signal transduction proteins in microbial genomes.
- To highlight new features and improvements in MiST2, enhancing its utility for researchers.
- To provide a comprehensive resource for exploring microbial signaling pathways.
Main Methods:
- Protein sequences are analyzed for specific domain profiles to identify signal transduction proteins.
- MiST2 incorporates novel domain models for histidine kinases and response regulators.
- The database includes draft genomes, extracytoplasmic function (ECF) sigma factors, and gene context information.
Main Results:
- MiST2 currently contains 966 complete and 157 draft bacterial and archaeal genomes.
- Over 245,000 signal transduction proteins have been identified and cataloged.
- Signal transduction proteins are predominantly one-component systems (66%), followed by two-component systems (26%).
Conclusions:
- MiST2 is a valuable, enhanced resource for studying microbial signal transduction.
- The database facilitates research into cellular activities, pathogen virulence, and antibiotic resistance.
- Expanded data and improved features in MiST2 support advancements in microbial genomics and biomedical discovery.
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