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Oleic acid induced pancreatitis in pigs.
B Vollmar1, H Waldner, J Schmand
1Department of Surgery, Innenstadt, Ludwigs-Maximilians-University of Munich, West Germany.
The Journal of Surgical Research
|February 1, 1991
Summary
Edematous pancreatitis in pigs significantly impairs pancreatic microcirculation and blood flow. This study reveals reduced capillary perfusion and altered enzyme and mediator levels, crucial for understanding pancreatitis progression.
Area of Science:
- Gastroenterology
- Surgical Research
- Physiology
Background:
- Edematous pancreatitis is a severe inflammatory condition affecting the pancreas.
- Understanding the microcirculatory and biochemical changes is vital for effective treatment strategies.
Purpose of the Study:
- To establish an experimental model of edematous pancreatitis in pigs.
- To investigate the effects of pancreatitis on pancreatic macro- and microcirculation, enzyme activity, and vasoactive mediators.
Main Methods:
- An in situ isolated pancreas model in pigs under general anesthesia.
- Videofluorescence microscopy for microcirculatory parameter measurement.
- Enzymatic assays for lipase and phospholipase A; radioimmunoassay for kallikrein, kininogen, and prostanoids.
Main Results:
- Pancreatitis induced by oleic acid led to significant decreases in pancreatic blood flow (-55%) and O2-consumption (-49%), with increased vascular resistance (+50%).
- Capillary perfusion decreased from 41% to 27% in perfused areas, with some areas showing persistent stasis.
- Elevated lipase, phospholipase A, kallikrein, and prostanoids (prostacyclin, thromboxane A2) were observed, alongside kininogen consumption.
Conclusions:
- The experimental model effectively replicates key features of edematous pancreatitis.
- Pancreatitis severely disrupts pancreatic microcirculation and activates inflammatory mediator systems.
- Findings highlight the complex interplay of circulatory dysfunction and biochemical alterations in pancreatitis.