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Related Experiment Video

Updated: Jun 18, 2026

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures
08:32

External Excitation of Neurons Using Electric and Magnetic Fields in One- and Two-dimensional Cultures

Published on: May 7, 2017

Two-dimensional nanosecond electric field mapping based on cell electropermeabilization.

Meng-Tse Chen1, Chunqi Jiang, P Thomas Vernier

  • 1Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA. mengtsec@usc.edu.

PMC Biophysics
|November 12, 2009
PubMed
Summary
This summary is machine-generated.

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Researchers mapped electric fields during nanoelectroporation of prostate cancer cells using fluorescence imaging. This method calibrates electrode designs for cell membrane permeabilization and cancer treatment applications.

Area of Science:

  • Biophysics
  • Cell Biology
  • Electroporation

Background:

  • Nanosecond, high-voltage electric pulses induce cell membrane permeabilization and apoptosis in tumor cells.
  • Electroporation is being explored as a potential treatment for skin cancer.
  • Accurate mapping of electric fields is crucial for optimizing electroporation therapies.

Purpose of the Study:

  • To construct 2D electric field maps during nanoelectroporation of PC3 prostate cancer cells.
  • To compare electric field distributions generated by three different electrode configurations.
  • To validate a live-cell fluorescence imaging method for measuring electric fields.

Main Methods:

  • Utilized nanoelectroporation with 15 ns, 10 kV pulses on PC3 cell monolayers.
  • Employed fluorescence imaging with YO-PRO-1 dye to indicate membrane permeabilization.

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Last Updated: Jun 18, 2026

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Published on: May 7, 2017

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  • Investigated single-needle, five-needle, and flat-cut coaxial cable electrode setups.

    • Main Results:

    • Fluorescence intensity correlated with applied electric field strength, indicating membrane permeabilization.
    • Spatial electric field distributions were successfully mapped in a plane near the electrode tip.
    • Experimental measurements showed good agreement with theoretical models for all tested electrode configurations.

    Conclusions:

    • Live-cell fluorescence imaging provides a reliable method for measuring nanosecond pulsed electric fields.
    • This technique allows for operational calibration of electrode designs for biological applications.
    • The method enables visualization of cell sensitivity to nanoelectropulse stimulation, aiding therapeutic development.