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Related Concept Videos

Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...

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High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
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An antibody surface for selective neuronal cell attachment.

Sanna Auer1, Riikka S Lappalainen, Heli Skottman

  • 1VTT Technical Research Centre of Finland, P.O. Box 1300, FIN-33101 Tampere, Finland. Sanna.Auer@vtt.fi

Journal of Neuroscience Methods
|November 12, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel surface using a neural cell adhesion molecule (NCAM) antibody to selectively culture human embryonic stem cell (hESC)-derived neuronal cells, enabling better cell attachment and viability.

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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Published on: June 29, 2016

Area of Science:

  • Neuroscience
  • Biotechnology
  • Stem Cell Biology

Background:

  • Culturing human embryonic stem cell (hESC)-derived neuronal cells requires optimal surfaces for efficient growth and differentiation.
  • Existing methods may lack selectivity, leading to challenges in isolating specific neuronal cell populations.

Purpose of the Study:

  • To develop and validate a selective surface for culturing hESC-derived neuronal cells using immobilized antibodies.
  • To investigate the attachment, viability, and morphology of hESC-derived neuronal cells on this novel surface.

Main Methods:

  • Immobilization of a specific neural cell adhesion molecule (NCAM) antibody onto a polystyrene surface.
  • Seeding of hESC-derived neurospheres and subsequent observation of individual neuronal cell attachment.
  • Assessment of cell viability and morphology over an 8-day period.
  • Surface plasmon resonance (SPR) measurements to confirm specific antigen-antibody binding.

Main Results:

  • The NCAM antibody-coated surface selectively promoted the attachment of individual neuronal cells while preventing neurosphere attachment.
  • Neuronal cell attachment was concentration-dependent on the immobilized NCAM antibody.
  • Neuronal cells remained viable and exhibited typical immature neuron morphology over 8 days.
  • SPR confirmed specific binding between the NCAM antigen and the antibody-coated surface.

Conclusions:

  • An antibody-based surface can serve as a selective matrix for culturing hESC-derived neuronal cells.
  • This approach offers a promising strategy for enhancing the selective isolation and culture of specific neuronal cell types.
  • This represents a novel method for directed neuronal cell culture using antibody immobilization.