Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging capabilities in microscale separations and bioanalysis.

Analytical and bioanalytical chemistry·2026
Same author

Quantifying and Minimizing the Variance of Gradient Insulator-Based Dielectrophoresis.

Micromachines·2026
Same author

Dielectrophoresis reveals stimulus-induced remodeling of insulin granule subpopulations.

Biophysical journal·2026
Same author

Dielectrophoresis Reveals Stimulus-Induced Remodeling of Insulin Granule Subpopulations.

bioRxiv : the preprint server for biology·2025
Same author

Streaming-Particle Method for Dielectrophoretic Characterization.

Electrophoresis·2025
Same author

Gradient Insulator-Based Dielectrophoresis of Gold Nanoparticles.

Electrophoresis·2025

Related Experiment Video

Updated: Jun 18, 2026

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
05:16

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Published on: July 26, 2021

Chapter 16 - Bionanotubules formed from liposomes.

Josemar A Castillo1, Mark A Hayes

  • 1Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona, USA.

Methods in Enzymology
|November 12, 2009
PubMed
Summary

Synthetic bionanotubules, lipid-bound structures, can be formed from liposomes using electric fields or micromechanical manipulation. These methods enable controlled extension of lipid bilayers into long, thin tubules for various applications.

More Related Videos

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
06:26

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Published on: December 7, 2017

Related Experiment Videos

Last Updated: Jun 18, 2026

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
05:16

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Published on: July 26, 2021

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
06:26

Pulling Membrane Nanotubes from Giant Unilamellar Vesicles

Published on: December 7, 2017

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Bionanotubules are naturally occurring or synthetically prepared lipid-bound cylindrical structures.
  • These structures possess nanoscale diameters and micrometer-scale lengths.
  • Lipid vesicles, or liposomes, serve as precursors for bionanotubule synthesis.

Purpose of the Study:

  • To describe methods for the synthetic formation of bionanotubules from liposomes.
  • To investigate the use of electric fields as a driving force for tubular extension.
  • To summarize various protocols for bionanotubule generation.

Main Methods:

  • Liposome preparation using lipid mixtures (phosphatidylcholine, phosphatidic acid, fluorescent phospholipids).
  • Surface attachment of liposomes followed by electric field-induced bilayer extension (<30V/cm).
  • Tubular extension from liposomes in solution using higher electric fields and micromechanical manipulation.

Main Results:

  • Demonstrated successful formation of lipid nanotubules from various liposome formulations.
  • Showcased electric fields as an effective method for controlled bionanotubule extension.
  • Presented a summary of diverse protocols for synthetic bionanotubule production.

Conclusions:

  • Synthetic bionanotubules can be reliably prepared using electric field-driven methods.
  • Multiple protocols exist for generating bionanotubules from liposomes, offering versatility.
  • These findings contribute to the understanding and application of nanostructured lipid materials.