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Related Concept Videos

Adsorption Isotherms I01:29

Adsorption Isotherms I

Adsorption isotherms are mathematical models that describe how molecules in a gas or liquid phase interact with surfaces. Two of the most common isotherm models are the Langmuir and Freundlich isotherms, which relate to Type I monolayer chemisorption. The Langmuir model is based on four key assumptions:• Adsorption cannot exceed monolayer coverage.• All surface sites are equivalent.• Molecules adsorb only at vacant sites.• There are no interactions between adsorbed molecules.Consider the...
Lipids as Anchors01:32

Lipids as Anchors

In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains the...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Adsorption Isotherms II01:25

Adsorption Isotherms II

Brunauer, Emmett, and Teller (BET) introduced a theory in 1938 that modified Langmuir's assumptions to explain multilayer physical adsorption. This theory is applicable to Type II isotherms and provides a more realistic picture of adsorption processes. The BET theory assumes a uniform solid surface with localized adsorption sites, where adsorption at one site doesn't affect adsorption at neighboring sites. This theory also allows for the possibility of additional molecules being adsorbed on top...
Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.Fatty acids tails of phospholipids can be either saturated or...

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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Protein adsorption and desorption on lipid bilayers.

Vladimir P Zhdanov1, Bengt Kasemo

  • 1Department of Applied Physics, Chalmers University of Technology, Göteborg, Sweden. zhdanov@catalysis.ru

Biophysical Chemistry
|November 12, 2009
PubMed
Summary

Protein adsorption to lipid bilayers is energetically favorable due to electrostatic interactions. However, lipid mobility and redistribution create a critical coverage point, hindering further protein adsorption kinetically and thermodynamically.

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Last Updated: Jun 18, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
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Area of Science:

  • Biophysics
  • Physical Chemistry
  • Materials Science

Background:

  • Protein adsorption to lipid bilayers is influenced by electrostatic interactions between charged protein surfaces and lipid head groups.
  • Lipid bilayers are dynamic systems with mobile lipids that can redistribute upon protein binding.

Purpose of the Study:

  • To develop a kinetic model for protein adsorption on lipid bilayers considering lipid mobility and redistribution.
  • To investigate the impact of charged lipid fractions on protein adsorption kinetics and thermodynamics.

Main Methods:

  • Development of a kinetic model simulating protein adsorption and desorption dynamics.
  • Analysis of the influence of protein coverage and charged lipid fraction on adsorption/desorption rate constants.

Main Results:

  • Protein adsorption is energetically favorable when charged lipids are present, due to electrostatic interactions.
  • Lipid redistribution significantly affects adsorption kinetics, especially at low charged lipid fractions.
  • A critical protein coverage fraction was identified, beyond which adsorption is hindered both kinetically and thermodynamically.

Conclusions:

  • Protein-lipid electrostatic interactions drive adsorption, but lipid mobility introduces complex kinetic and thermodynamic constraints.
  • The dynamic redistribution of lipids plays a crucial role in regulating protein adsorption on bilayers.
  • Understanding these kinetic and thermodynamic limitations is essential for predicting and controlling protein-bilayer interactions.