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Updated: Jun 18, 2026

mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development
Xin-Jian He1, Yi-Feng Hsu, Shihua Zhu
1Institute for Integrative Genome Biology and Department of Botany and Plant Sciences, University of California at Riverside, Riverside, California 92521, USA.
Abstract:
RNA-directed DNA methylation (RdDM) is a conserved mechanism for epigenetic silencing of transposons and other repetitive elements. We report that the rdm4 (RNA-directed DNA Methylation4) mutation not only impairs RdDM, but also causes pleiotropic developmental defects in Arabidopsis. Both RNA polymerase II (Pol II)- and Pol V-dependent transcripts are affected in the rdm4 mutant. RDM4 encodes a novel protein that is conserved from yeast to humans and interacts with Pol II and Pol V in plants. Our results suggest that RDM4 functions in epigenetic regulation and plant development by serving as a transcriptional regulator for RNA Pol V and Pol II, respectively.
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