Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pinching-off of Coated Vesicles

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...

Vesicular Tubular Clusters

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

Types of Membrane Protrusions

Types of Membrane Protrusions

The protrusion of the cell surface is an initial step for several cellular processes, including cell migration, phagocytosis, and neurite outgrowth. These membrane protrusions are a result of cytoskeletal rearrangement. The most widely observed cell protrusions include lamellipodia, pseudopodia, filopodia, microvilli, invadopodia, and podosomes. These protrusions can be of two types — static or dynamic.
The microvilli, an example of stable protrusions, are finger-like projections with a...

Cell Inclusions

Cell Inclusions

Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid polymers that...

Intralumenal Vesicles and Multivesicular Bodies

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...

Cleavage and Blastulation

Cleavage and Blastulation

After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Directional motion of a self-steering active intruder in a dense crowd of cognitive active agents.

Scientific reports·2026

Same author

Rosette margination in blood flow during malaria pathogenesis.

Biophysical journal·2025

Same author

Reversible formation of von-Willebrand-factor-platelet aggregates in microvascular blood flow.

PNAS nexus·2025

Same author

Tunable colloidal swarmalators with hydrodynamic coupling.

Nature communications·2025

Same author

Wrapping of Nano- and Microgels by Lipid-Bilayer Membranes.

ACS macro letters·2025

Same author

Polymeric and Polymer-Functionalized Drug Delivery Vectors: From Molecular Architecture and Elasticity to Cellular Uptake.

Polymers·2025

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

Budding and vesiculation induced by conical membrane inclusions.

Thorsten Auth¹, Gerhard Gompper

¹Institut für Festkörperforschung and Institute for Advanced Simulations, Forschungszentrum Jülich, D-52425 Jülich, Germany.

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics

|November 13, 2009

Summary

This summary is machine-generated.

Conical inclusions in lipid bilayers drive membrane budding and vesiculation by aggregating at high densities. The model predicts bud radii and reveals how bending energy and translational entropy influence vesicle size.

More Related Videos

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

Related Experiment Videos

Last Updated: Jun 18, 2026

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

Published on: August 5, 2021

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

Area of Science:

Biophysics
Membrane biology
Soft matter physics

Background:

Conical inclusions like proteins, viruses, and lipid domains induce spontaneous curvature in lipid bilayers.
Membrane curvature is crucial for processes such as budding and vesiculation.

Purpose of the Study:

To develop an analytical model for lipid bilayer budding and vesiculation driven by conical inclusions.
To investigate the roles of membrane bending energy and translational entropy in these processes.

Main Methods:

Analytical modeling based on membrane bending energy and inclusion translational entropy.
Comparison of model predictions with existing computer simulations.

Main Results:

High inclusion density leads to aggregation, bud formation, and vesiculation.
The model predicts bud radii using bending energy alone.
Bending energy and translational entropy have competing effects on vesicle size during splitting.

Conclusions:

The interplay between bending energy and translational entropy governs lipid bilayer vesiculation.
The model provides a framework for understanding membrane shape transformations induced by inclusions.