Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromosome Replication

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins. This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

The DNA Replication Fork

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork. Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

RGX-019-MMAE inhibits leukemia progression by targeting MER proto-oncogene tyrosine kinase (MERTK) in acute myeloid leukemia.

Journal of experimental & clinical cancer research : CR·2026

Same author

A MERTK-Targeting Antibody-Drug Conjugate Selectively Depletes M2 Tumor-Associated Macrophages and MERTK-Expressing Cancer Cells.

Cancer research·2026

Same author

Carboxylic Acid Bioisosteres of Creatine as Novel and Selective Substrate Competitive Inhibitors of the Creatine Transporter SLC6A8.

Journal of medicinal chemistry·2026

Same author

Tumor Genotype Dictates Mitochondrial and Immune Vulnerabilities in Liver Cancer.

bioRxiv : the preprint server for biology·2025

Same author

UVSSA facilitates transcription-coupled repair of DNA interstrand crosslinks.

DNA repair·2024

Same author

Replication-Independent ICL Repair: From Chemotherapy to Cell Homeostasis.

Journal of molecular biology·2024

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Origin-dependent initiation of DNA replication within telomeric sequences.

Isabel Kurth¹, Jean Gautier

¹Institute for Cancer Genetics, Department of Genetics and Development and Herbert Irving Comprehensive Cancer Center, Columbia University Medical Center, New York, NY 10032, USA.

Nucleic Acids Research

|November 13, 2009

Summary

This summary is machine-generated.

Vertebrate telomeres can initiate DNA replication. Researchers observed pre-replicative complex assembly and origin-dependent replication within telomeric DNA, suggesting telomeres can function as replication origins.

More Related Videos

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Related Experiment Videos

Last Updated: Jun 18, 2026

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Telomere replication is crucial for maintaining chromosome stability.
The ability of vertebrate telomeres to initiate DNA replication remains experimentally unaddressed.

Purpose of the Study:

To investigate whether vertebrate telomeres can support the initiation of DNA replication.
To determine if pre-replicative complex proteins assemble at telomeric DNA and if replication can be initiated.

Main Methods:

Utilized Xenopus cell-free extracts to establish a system for studying replication initiation on linear telomeric DNA substrates.
Analyzed the binding of TRF2 (telomere repeat-binding factor 2) to telomeric DNA.
Observed the assembly of pre-replicative complex (ORC2, MCM6, Cdc6) proteins and replication initiation under conditions inhibiting checkpoint activation.

Main Results:

TRF2 binds to telomeric DNA, indicating recognition by shelterin components.
Assembly of pre-replicative complex proteins (ORC2, MCM6, Cdc6) onto telomeric DNA was observed.
Origin-dependent replication of telomeric substrates was detected, even when checkpoint activation was inhibited.

Conclusions:

Telomeric DNA is recognized by shelterin components.
Pre-replicative complexes can assemble on telomeric DNA.
Telomeric DNA can function as a replication origin, supporting origin-dependent replication.