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Histamine stimulates interleukin-6 production through histamine H1 receptors in human amnion cells
Akiko Morimitsu1, Seiji Sumigama, Tomomi Kotani
1Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Background/Aims:
Previous studies have stated that maternal allergic diseases are associated with increased risk of preterm labor/delivery, but the underlying mechanisms remain unclear. This study tested the hypothesis that histamine induces interleukin (IL)-6 production in amnion cells.
Methods:
Using cultured human amnion cells, we examined expression of histamine receptors and effects of histamine on IL-6 production.
Results:
Reverse transcription-polymerase chain reaction and Western blotting revealed expression of histamine H1 receptor (H1R) and H2 receptor (H2R) in human amnion. Histamine stimulation significantly increased concentrations of IL-6 in conditioned medium, as did tumor necrosis factor-alpha and IL-1beta in positive controls. In addition, the H1R antagonist olopatadine significantly blocked histamine-induced production of IL-6, whereas the H2R antagonist ranitidine did not.
Conclusion:
Histamine appears to induce IL-6 production through H1R in human amnion cells.
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