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Updated: Jun 18, 2026
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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
Published on: May 9, 2019
Gene expression profiling of mouse epidermal keratinocytes.
Ramón García-Escudero1, Jesús M Paramio
1Molecular Oncology Unit, Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Madrid, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2009
Summary
This review details DNA microarray methods for analyzing gene expression in mouse skin and keratinocytes. It covers RNA handling, quality control, microarray types, and data analysis for gene deregulation insights.
Area of Science:
- Molecular Biology
- Genomics
Background:
- DNA microarray technology enables comprehensive genome-wide gene expression profiling.
- Skin and epidermal keratinocytes are key cell types for studying skin biology and disease.
Purpose of the Study:
- To provide a comprehensive review of DNA microarray methodology for gene expression analysis in mouse skin and keratinocytes.
- To outline protocols for RNA handling, quality assessment, and microarray data analysis.
Main Methods:
- Review of established protocols for RNA preservation, purification, and integrity testing.
- Description of various DNA microarray technologies applicable to skin samples.
- Explanation of data preprocessing, normalization, differential expression analysis, and functional interpretation.
Main Results:
- Detailed methodology for conducting gene expression profiling in mouse skin and keratinocytes using DNA microarrays.
- Step-by-step guide for raw data processing, normalization, and statistical analysis.
- Framework for gene clustering and functional analysis to understand gene deregulation.
Conclusions:
- DNA microarray technology is a robust tool for investigating gene expression patterns in skin and keratinocytes.
- Standardized protocols and rigorous data analysis are crucial for reliable gene expression profiling.
- This review serves as a valuable resource for researchers utilizing microarrays in skin biology studies.
