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Cytokine release in tissue-engineered epidermal equivalents after prolonged mechanical loading
Lisette H Cornelissen1, Debbie Bronneberg, Susan Gibbs
1Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2009
Summary
Measuring cytokine release from engineered skin can assess tissue response to mechanical loading, potentially improving pressure ulcer risk assessment. This study details methods for quantifying specific cytokines like IL-1 alpha, IL-1RA, and IL-8.
Area of Science:
- Biomedical Engineering
- Tissue Engineering
- Dermatology
Background:
- Prolonged mechanical loading can cause soft tissue degeneration and pressure ulcers.
- Assessing tissue response to mechanical stress is crucial for risk evaluation.
- Cytokines released by skin upon irritation indicate epidermal response.
Purpose of the Study:
- To describe methods for measuring cytokine release from tissue-engineered epidermal equivalents.
- To investigate the epidermal response to sustained mechanical loading.
- To quantify specific cytokines: IL-1 alpha, IL-1RA, and IL-8.
Main Methods:
- Utilized tissue-engineered epidermal equivalents.
- Applied sustained mechanical loading using a custom-built device.
- Measured cytokine release (IL-1 alpha, IL-1RA, IL-8) via enzyme-linked immunosorbent assay (ELISA).
Main Results:
- Established a method to quantify cytokine release from engineered epidermis under mechanical load.
- Demonstrated the feasibility of measuring IL-1 alpha, IL-1RA, and IL-8 release.
- Provided a basis for further research into mechanical loading effects on skin.
Conclusions:
- Cytokine release measurement is a viable method to assess epidermal response to mechanical loading.
- This approach may enhance pressure ulcer risk assessment strategies.
- Further studies can refine these methods for clinical application.
