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Related Experiment Video

Updated: Jun 18, 2026

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

Three-dimensional human tissue models of wounded skin.

Christophe Egles1, Jonathan A Garlick, Yulia Shamis

  • 1Division of Cancer Biology and Tissue Engineering, Department of Oral and Maxillofacial Pathology, School of Dental Medicine, Sackler Graduate School and School of Engineering, Tufts University, Boston, MA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2009
PubMed
Summary

Human skin equivalents (HSEs) are engineered in vitro skin models. These models mimic human skin for studying wound repair and epithelial tissue healing processes.

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Area of Science:

  • Tissue engineering
  • Dermatology
  • Regenerative medicine

Background:

  • Human skin equivalents (HSEs) are in vitro models comprising a stratified squamous epithelium on a collagen gel with fibroblasts.
  • HSEs serve as experimental models to investigate re-epithelialization and epithelial-mesenchymal interactions post-wounding.

Purpose of the Study:

  • To describe the fabrication of HSEs using human keratinocytes and fibroblasts.
  • To detail methods for modifying HSEs to assess epithelial responses during wound repair.
  • To present protocols for generating human skin models that replicate native skin architecture and differentiation.

Main Methods:

  • Fabrication of HSEs from human keratinocytes and dermal fibroblasts.
  • Culturing of stratified squamous epithelium at an air-liquid interface on a Type I collagen gel.

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Last Updated: Jun 18, 2026

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

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Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

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The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression

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  • Adaptation of HSEs to monitor epithelial response following induced wounding.

    • Main Results:

    • Protocols enable the generation of engineered human tissues closely approximating native skin.
    • Developed methods allow for the characterization of epithelial responses in HSEs after wounding.
    • HSEs provide a robust platform for studying wound healing mechanisms.

    Conclusions:

    • HSEs are valuable in vitro tools for studying skin biology and wound healing.
    • Engineered human skin models facilitate research into epithelial-mesenchymal cross-talk.
    • These models offer insights into the biological processes of human skin and epithelial tissue repair.