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Related Experiment Video

Updated: Jun 18, 2026

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

Functionally defining the endothelial transcriptome, from Robo4 to ECSCR.

Ana Raquel Verissimo1, John M J Herbert, Victoria L Heath

  • 1Institute for Biomedical Research, Birmingham University Medical School, Vincent Drive, Birmingham B15 2TT, UK.

Biochemical Society Transactions
|November 14, 2009
PubMed
Summary
This summary is machine-generated.

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Identifying endothelial cell genes like Robo4 and ECSCR is key for understanding blood vessel formation. These genes are crucial for cell migration and angiogenesis, impacting blood vessel development.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Vascular Biology

Background:

  • Endothelial cells are vital for angiogenesis and blood vessel formation.
  • Specific genes expressed by endothelial cells play critical roles in their function.
  • Identifying these genes can reveal new therapeutic targets for vascular diseases.

Purpose of the Study:

  • To identify and characterize endothelial cell-specific genes involved in cell migration and angiogenesis.
  • To investigate the roles of roundabout receptor Robo4 and endothelial-cell-specific chemotaxis regulator (ECSCR) in endothelial cell function.

Main Methods:

  • Utilized search algorithms on expression databases to find endothelial cell-specific genes.
  • Employed siRNA to knock down Robo1 and Robo4 expression in endothelial cells.

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Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
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Last Updated: Jun 18, 2026

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level
09:45

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

Published on: March 14, 2022

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
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Published on: May 25, 2019

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  • Conducted in vitro assays, including Matrigel tube formation and filopodial extension analysis.
  • Performed immunoprecipitation and yeast two-hybrid assays to study protein interactions.

    • Main Results:

    • Knockdown of Robo4 and Robo1 inhibited endothelial cell tube formation and migration.
    • Robo4 transfection increased filopodial extensions, dependent on Robo1.
    • Robo1 and Robo4 were found to heterodimerize and signal through WASP.
    • ECSCR knockdown impaired endothelial cell chemotaxis and tube formation.
    • ECSCR interacts with filamin A, distinct from Robo protein interactions.

    Conclusions:

    • Robo1, Robo4, and ECSCR are critical for endothelial cell migration and angiogenesis.
    • The Robo1-Robo4 heteroduplex regulates filopodia formation and cell migration via WASP.
    • ECSCR mediates endothelial cell chemotaxis and tube formation through interaction with filamin A.
    • These identified genes represent potential targets for modulating angiogenesis.