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Updated: Jun 18, 2026

A High-content Assay for Monitoring AMPA Receptor Trafficking
Published on: January 28, 2019
Ca2+-dependent modulation of GABAA and NMDA receptors by extracellular ATP: implication for function of tripartite
Ulyana Lalo1, Jemma Andrew, Oleg Palygin
1Department of Cell Physiology and Pharmacology, Henry Wellcome Building 2/33, University of Leicester, Leicester LE1 9HN, UK.
Abstract:
The importance of communication between neuronal and glial cells for brain function is recognized by a modern concept of 'tripartite synapse'. Astrocytes enwrap synapses and can modulate their activity by releasing gliotransmitters such as ATP, glutamate and D-serine. One of the regulatory pathways in the tripartite synapse is mediated by P2X purinoreceptors. Release of ATP from synaptic terminals and astrocytes activates Ca(2+) influx via P2X purinoreceptors which co-localize with NMDA (N-methyl-D-aspartate) and GABA (gamma-aminobutyric acid) receptors and can modulate their activity via intracellular cascades which involve phosphatase II and PKA (protein kinase A).
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