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[Effect of different salts and their concentration on polymyxin M biosynthesis by Bac. polymyxa Ross]
Abstract:
The effect of various concentrations of ammonium sulfate, sodium and potassium chlorides and sulfates on biosynthesis of polymyxin M by Bac. polymyxa Ross. on media containing wheet meal as the carbon source was studied. The culture growth, antibiotic accumulation and consumption of the main nutrients was studied as dependent on the level of the salts in the medium. The highest productivity was observed on media with the salt concentrations amounting to 1--2 per cent except sodium sulfate: changes in the concentration of the latter had practically no effect on the activity of the fermentation broth.
Insights
Salt concentrations significantly impact polymyxin M biosynthesis by Bac. polymyxa. Optimal productivity for this antibiotic was achieved with 1-2% salt concentrations, excluding sodium sulfate.
Area of Science:
- Microbiology
- Biotechnology
- Biochemical Engineering
Background:
- Bacillus polymyxa produces the antibiotic polymyxin M.
- Polymyxin M is a crucial antibiotic with significant therapeutic applications.
- Optimizing antibiotic biosynthesis is vital for industrial production.
Purpose of the Study:
- To investigate the impact of various salt concentrations on polymyxin M biosynthesis.
- To determine the optimal salt concentrations for Bac. polymyxa growth and antibiotic production.
- To understand the relationship between nutrient consumption and salt levels during fermentation.
Main Methods:
- Culturing Bac. polymyxa Ross. on wheat meal-based media.
- Supplementing media with varying concentrations of ammonium sulfate, sodium chloride, potassium chloride, and sodium sulfate.
- Monitoring culture growth, antibiotic accumulation, and nutrient consumption.
Main Results:
- Ammonium sulfate, sodium chloride, and potassium chloride at 1-2% concentrations enhanced polymyxin M biosynthesis.
- Sodium sulfate concentration had a negligible effect on antibiotic production.
- Optimal salt concentrations promoted higher antibiotic yields and efficient nutrient utilization.
Conclusions:
- Specific salt concentrations are critical for maximizing polymyxin M production.
- Bacillus polymyxa fermentation is sensitive to inorganic salt levels, with notable exceptions like sodium sulfate.
- Further research can refine media composition for enhanced antibiotic fermentation.