Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanistic evaluation of Curcumae rhizoma-Atractylodis macrocephalae rhizoma herb pair enhancing anti-chronic hepatic injury through PI3K/AKT/mTOR signaling pathway.

Journal of ethnopharmacology·2026
Same author

A chromosome-level genome assembly and multi-omics analysis reveal fenpropathrin resistance mechanisms in the turnip aphid, Lipaphis erysimi.

Pest management science·2026
Same author

Colony Binary Classification Based on Persistent Homology Feature Extraction and Improved EfficientNet.

Bioengineering (Basel, Switzerland)·2025
Same author

Profiling electric signals of electrogenic probiotic bacteria using self-attention analysis.

Applied microbiology and biotechnology·2025
Same author

Incidence trends and projections of lip and oral cavity cancer in China 1990-2021: an age-period-cohort and decomposition analysis.

BMC oral health·2025
Same author

EEG Channel Localization and Selection via Training with Noise Injection for BCI Applications.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025

Related Experiment Video

Updated: Jun 18, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Mass spectrometry-based label-free quantitative proteomics.

Wenhong Zhu1, Jeffrey W Smith, Chun-Ming Huang

  • 1Center on Proteolytic Pathways, Burnham Institute for Medical Research, 10901 N. Torrey Pines Road, La Jolla, CA 92037, USA.

Journal of Biomedicine & Biotechnology
|November 14, 2009
PubMed
Summary
This summary is machine-generated.

Label-free shotgun proteomics offers faster, simpler protein quantification than isotope or fluorescent labeling. This review covers label-free methods, statistics, software, and applications in complex biological samples.

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Related Experiment Videos

Last Updated: Jun 18, 2026

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors
10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

Published on: April 29, 2022

Area of Science:

  • Proteomics
  • Mass Spectrometry
  • Quantitative Biology

Background:

  • Accurate protein quantification is crucial for studying differential protein expression in complex biological samples.
  • Traditional isotope and fluorescent labeling methods are widely used but can be complex.
  • Label-free techniques are emerging as a preferred alternative for faster, simpler, and cleaner results.

Purpose of the Study:

  • To review label-free quantitative proteomics techniques.
  • To discuss statistical approaches and computational software for label-free analysis.
  • To highlight the applications of these methods in complex proteomics studies.

Main Methods:

  • Label-free quantitative proteomics using mass spectrometry.
  • Methods include chromatographic ion intensity measurements (e.g., peptide peak areas/heights).
  • Methods also include spectral counting of identified proteins.

Main Results:

  • Label-free methods offer advantages in speed, simplicity, and reproducibility.
  • Two main categories of label-free quantification exist: ion intensity-based and spectral counting-based.
  • Various statistical and computational tools are available to support these methods.

Conclusions:

  • Label-free shotgun proteomics is a powerful approach for quantitative proteomics.
  • The discussed methods, statistics, and software enable robust analysis of complex proteomes.
  • These techniques are increasingly vital for advancing biological research.